- Overview
- Protocols
- Specifications
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Obtain Higher Yields of cDNA than with the Deletion Mutant
Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus (M-MLV RT [H–]), Point Mutant, is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long RNA templates (>5kb). The lack of RNase H activity is beneficial for this application, as RNase H can start to degrade templates when incubation times are long, as they may be when synthesizing long cDNAs. Although many researchers are successful in using M-MLV RT (H+) for analytical and some preparative cDNA applications, reverse transcriptases lacking RNase H activity provide another option to prepare long cDNAs and libraries containing a high percentage of full-length cDNA.
Advantages
- RNase H Minus: Provides optimal conditions to prepare full-length cDNA from long RNA templates.
- Temperature Stability: Thermostability of this point mutant minimizes problems associated with secondary structure.
- Increased Polymerase Activity: M-MLV RT (H–), Point Mutant, gives higher yields of cDNA compared with the deletion mutant (Cat.# M5301).
- Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl, 15mM MgCl2, 50mM DTT.
- Broad Working Range: More tolerance to variations in enzyme and substrate concentrations means improved consistency in performance.
- First-strand cDNA synthesis.
- Primer extension.
Thermal stability and synthesis of large transcripts. -
Protocols
Complete Protocol
M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant Protocol
PDF (111 KB)
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Certificate of Analysis
Lookup Certificate of AnalysisStorage Conditions
-30C TO -10C
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
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Resources
Articles
- Choosing the Right Reverse Transcriptase
- M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant: A Cost-Effective Alternative to SuperScript® II
- Use of M-MLV RT, RNase H-, Point Mutant, for mRNA-Differential Display Analysis of Parathyroid Hormone-Related Peptide (PTHrP)-Treated Breast Carcinoma Cells
Citations
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Biology of Zika Virus Infection in Human Skin Cells
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2015 J. Virol.