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Monster Green Fluorescent Protein phMGFP Vector

by Promega
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  • Overview
  • Protocols
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  • The phMGFP Vector contains the open reading frame for the Monster Green® Fluorescent Protein cloned into a mammalian expression vector. The Monster Green® Fluorescent Protein is encoded by an improved synthetic version of the green fluorescent protein gene originally cloned from Montastrea cavernosa (Great Star Coral). The synthetic gene (hMGFP) expresses a 26kDa protein that shows improved fluorescence intensity compared to the native gene. Furthermore, the hMGFP gene has been codon optimized and cleared of most consensus sequence transcription factor binding sites to ensure reliability and high levels of expression.

    The Monster Green® Fluorescent Protein encoded by the hMGFP gene is an ideal fluorescent reporter, providing high-level fluorescence and reducing cytotoxicity. Monster Green® Fluorescent Protein generally fluoresces at least 20% brighter than other commercially available green fluorescent proteins (GFPs) and also minimizes cellular perturbations, offering flexibility when working with transient and stable expression assays.

    3898MA11_2A-W

    Monster Green® Fluorescent Protein phMGFP Vector GenBank® Accession Number AY218848 and vector sequence text file.

  • Protocols

    Complete Protocol

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    Monster Green® Fluorescent Protein phMGFP Vector Technical Bulletin

    PDF (262 KB)

  • Certificate of Analysis

    Lookup Certificate of Analysis

    Storage Conditions

    -30C TO -10C

    Use Restrictions

    For Research Use Only. Not for Use in Diagnostic Procedures.

  • Resources

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