mspi

MspI

by Promega

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Overview
Protocols
Specifications
Resources

Cut Site:

C^?CG G
G GC_?C

Note: MspI and its isoschizomer HpaII exhibit different methylation sensitivities.

Incubation Conditions: Buffer B. 37°C.

Source: Moraxella sp.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

A B C D MULTI-CORE™

75–100% 100% 75–100% 25–50% 25–50%

Frequency of Cutting:

? Ad-2 ?X174 pUC18 M13mp18 pBR322

328 171 5 13 18 26
Protocols

Complete Protocol

Restriction Enzyme Protocol

PDF (410 KB)
Certificate of Analysis
Lookup Certificate of Analysis
Storage Conditions

-30C TO -10C

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.
Resources

Citations
- General suppression of Escherichia coli O157:H7 in sand-based dairy livestock bedding.
  --
  2011 Appl. Environ. Microbiol.
Other
- Restriction Enzyme Resource