ReliaPrep RNA Clean-Up and Concentration System
The purity and concentration of RNA prepared from conventional isolation protocols (organic extraction or column-based procedures) is often affected by carryover of purification reagents like guanidine salts and ethanol. Furthermore, the requirements of some purification kits for large elution volumes can leave the user with RNA too dilute for some downstream applications. Additionally, a simpler and faster protocol for enzymatic RNA reactions (reverse transcription or post-DNase treatment) is also desired. The ReliaPrepª RNA Clean-up and Concentration System was designed to address these needs while maintaining good recovery of RNA samples and ensuring their usefulness in downstream applications such as RT-qPCR, Northern blot analysis or RNA sequencing. An added benefit is that dilute samples or sample pools up to 300µl can be processed over a single column and eluted in 15µl of water or TE buffer. The system can process up to 12 samples in less than 10 minutes.