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Janelia Fluor 646 HaloTag Ligand

by Promega
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The Janelia Fluor 549 and Janelia Fluor 646 HaloTag Ligands enable fluorescent labeling, confocal and high-resolution single-molecule imaging studies of HaloTag fusion proteins in living cells. The Janelia Fluor dyes are the result of a simple substitution of a N,N-dimethylamino group with an azetidine ring, resulting in improved quantum yield and photostability. Additionally, the enhanced brightness of these dyes allows for use in detection and imaging of endogenous proteins fused to HaloTag, Fluorescence Activated Cell Sorting at low levels of expression and in-gel detection of fusion proteins using a fluorimager.