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ReliaPrep DNA Clean-Up and Concentration System

by Promega
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The ReliaPrepª DNA Clean-up and Concentration System is designed to quickly concentrate and purify dilute DNA solutions, extract and purify DNA fragmensts of 100bpÑ10kb from standard or low-melt agarose gels in either Tris acetate (TAE) or Tris borate (TBE) buffer, and to purify products directly from a PCR amplification. Up to 95% recovery is achieved depending on the DNA fragment size. This membrane-based system, which can bind up to 60µg of DNA , concentrates as much as 300µl of dilute DNA, recovering isolated DNA fragments or PCR products in as little as 10 minutes. The purified DNA can be used for automated fluorescent DNA sequencing, cloning, labeling, restriction enzyme digestion, next-generation sequencing or in vitro transcription/translation. The ReliaPrepª DNA Clean-up and Concentration System can be used with linear DNA fragments, supercoiled plasmid DNA or single-stranded linear or circular DNA.