AKT1 is a serine-threonine protein kinase that is activated by Platelet-Derived Growth Factor (PDGF) and PI 3-kinase signaling pathways. AKT has been shown to be a critical mediator of survival and apoptosis signaling events. Activation of AKT1 involves (and requires) phosphorylation of serine 473. AKT is also referred to as Protein Kinase B (PKB) and RAC. Recombinant AKT1 protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_005154.2) with an N-terminal 6xHis and FLAG tag. The molecular weight of the protein is 60.5 kDa.