Universal Magnetic Co-IP Kit
Active Motif's Universal Magnetic Co-IP Kit improves co-immunoprecipitation (Co-IP) through the use of protein G-coated magnetic beads, which speed and simplify the procedure while greatly reducing background. The kit includes optimized reagents for making both nuclear & whole-cell extracts from cells or tissue, giving you the flexibility to Co-IP any protein complex, whether it was originally bound to DNA or in the cytoplasm. To further improve Co-IP of nuclear complexes, the kit's proprietary Enzymatic Shearing Cocktail uses DNA digestion to release undissociated protein complexes from the DNA. Even histone complexes, which are not released from the DNA by traditional high-salt extraction methods, can be studied.