Research in cell free circulating DNA (ccfDNA) has become a key area of interest in the study of normal and disease states. In this first of three ccfDNA webinars, we discuss the history and basics of ccfDNA – what is it, how do laboratories commonly extract ccfDNA, and what are typical applications for ccfDNA?
Circulating cell-free DNA (ccfDNA) found in plasma and other bodily fluids shows great promise as a source of easily accessible DNA-based information. Research is giving rise to the possibility of liquid biopsies that would eliminate the need for invasive tissue collection. We will discuss ccfDNA in relation to the other molecules commonly referred to as liquid biopsy. When working with ccfDNA there are many considerations and we will discuss factors for sample collection from blood, expected recovery (yields) of ccfDNA and thoughts on the detection of rare events given expected yields and the frequency of mutational events vs. the sensitivity of various downstream assays.
- Doug White graduated from University of Wisconsin Madison with a Master’s Degree in Biochemistry and has been a Promega scientist for 25 years. While at Promega, he’s developed multiple key products including PepTag®, IsoQuant®, PureYield™ Midi and Maxipreps, and various ReliaPrep™ Nucleic Acid Purification Kits.
Select from three different times for the webinar here: Tuesday, February 13, 2018