Precision Variant Library Technology Allows for Focused Screening

Here we highlight Twist Bioscience’s ability to construct complex, high diversity libraries at confined regions (for example, CDRs) and introduce technology advancements that enable construction of synthetic DNA libraries with diversity scattered along the length of constructs. We demonstrate the precision of this technology through next-generation DNA sequencing validation. This technology provides a powerful solution for focusing libraries to precisely interrogate the variant space and enables users to make iterative designs that hone in the target.