Making Improvements in Life Possible
QIAGEN need little introduction, serving more than 500,000 customers around the globe, all seeking answers from the building blocks of life – DNA, RNA and proteins.
They deliver successful Sample to Insight solutions for molecular testing, propelling customers from start to finish to unlock new insights, making improvements in life possible.
QIAGEN provide differentiating Sample to Insight solutions that help customers to unlock valuable molecular insights encoded in the building blocks of life.
Scientists, doctors, lab directors and other professionals committed to answering life’s questions look to QIAGEN for solutions in molecular testing.
The power of digital PCR – finding a needle in a haystack
Digital PCR (dPCR) is a technique where the sample is partitioned into many individual reactions so that either zero, one or more target molecules are present in each reaction. This approach makes it surprisingly easy to detect a single positive against a strong background of negatives, a typical needle in a haystack problem. It is in such instances that the lower limit of detection excludes standard real-time quantitative PCR (qPCR) as a viable method.
The defining feature of digital PCR is the absolute quantification of nucleic acids. After partitioning, the reactions undergo end-point PCR cycling, and partitions are analyzed for the presence (positive reaction) or absence (negative reaction) of a fluorescent signal. Based on this information, one can calculate the absolute number of molecules present in the sample. Unlike qPCR, dPCR does not rely on standard curves. Thanks to this, dPCR has a lower detection limit and higher precision than qPCR.
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