Trusted, accurate and safe controls for a wide-range of applications

The recent Coronavirus pandemic has led to the unprecedented need for diagnostic tests for detecting the presence of SARS-CoV-2 and other Respiratory viruses in a variety of sample types. To address this need, laboratories around the world need high quality tools to enable them in catering to this rapidly expanding requirement for testing.

Fully synthetic RNA

  • Includes fully synthetic RNA generated from Twist gene fragments
  • Powerful alternative to live viruses

Full viral genome coverage

  • >99.9% viral genome coverage
  • NGS sequence verified
  • Enables versatility in assay design

Versatile controls

  • Positive controls perform for both qPCR and NGS assays
  • Content is well-aligned with Twist target enrichment panels

Monkeypox Controls

Twist’s Synthetic human Monkeypox Virus (hMPXV) Controls are based on sequences from either the Congo Basin (CB) or West African (WA) clades of hMPXV and provide coverage of ≥80% of the viral genome. As a result, these controls support the design of custom assays targeting regions of the genome relevant to orthopoxvirus research and can be used for both amplicon and capture-based detection methods.


SARS-CoV2 Controls

Twist has created synthetic RNA controls for several viral genomes. These variants were selected to cover a wide range of sequence diversity. Each control consists of six non-overlapping 5 kb fragments generated from Twist Gene Fragments then transcribed into ssRNA. These controls provide coverage of greater than 99.9% of the bases of the SARS-CoV-2 viral genome.


Respiratory Virus Controls

The Twist Synthetic Respiratory Virus Controls cover a broad range of RNA and DNA viruses relevant to respiratory disease research. These controls are aligned with the content of the Twist Respiratory Virus Research Panel (PN 103067) and are suitable for use with Twist Fixed Panel NGS workflows or for use with RT-PCR or qPCR experiments of your own design.


Reproducible, Quality Results

Positive controls provide quality control measures for a wide range of applications from diagnostic assay development to day-to-day testing. This includes the verification and validation of diagnostic tests of both next-generation sequencing (NGS) and reverse transcription polymerase chain reaction (RT-PCR) assays.

A Safe and Effective Alternative to “Live Virus”

Controls based on viral nucleic acids extracted from either an infected patient or from live virus propagated in cell culture have safety and security concerns. Synthetic controls created through gene synthesis broaden access across diverse strains while mitigating risk.

Flexible formats for your applications

Choose from Standard, Assay Ready, or Encapsuled Control formulations. The standard format provides the controls at high concentration in a frozen liquid formulation. The Assay Ready Controls are provided as a dried pellet that increases stability. Just as protein encapsulation of viruses offers more stable molecular storage, Twist’s The Encapsulated Control products leverage a metal capsule sealed around a desiccated pellet to enhance stability of the RNA controls.

Both the Assay Ready and the Encapsulated formulations are single use controls. The Assay Ready format allows for shipping at ambient temperatures and storage in standard freezers. The enhanced stability of the Encapsulated controls enables extended shelf life and both ship and store at room temperature. Ambient shipping reduces cost and increases accessibility for researchers across the globe.

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