Twist Webinar | Connecting Plant Trait Genotypes to Phenotypes using WGS Skim-Seq


  • High-quality genetic maps can be efficiently built with whole-genome skim sequencing
  • Genetic maps and QTL analysis can be used to identify genomic regions responsible for phenotype
  • Twist library prep makes screening genetic markers in many samples efficient and cost-effective

One of the primary applications of genomics is to connect genotype to phenotype. Linkage mapping utilizes recombination to identify regions of the genome that code for traits of interest. Our lab has used the cost-effective high-throughput Twist 96-Plex Library Prep (Riptide) kit in multiple crosses to perform whole-genome skim sequencing and create high-quality genetic maps. These genetic maps have been used to identify regions of the genome and, using quantitative trait locus (QTL) analysis, specific candidate genes responsible for various plant traits. I will demonstrate our process from raw genomic DNA to a genetic map and QTL. This process can be applied to and adapted for any diploid system.

*Results are specific to the institution where they were obtained and may not reflect the results achievable at other institutions.