FACE™ MEK1/2 Chemi

by Active Motif
Available SKUs: 48780 |
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Description

Fast Activated Cell-based ELISA (FACE™) Kits provide a simple, sensitive method for detecting protein phosphorylation directly in the cell, without making extracts or performing electrophoresis and membrane blotting. These 96-well, high-throughput assays are available in both colorimetric and chemiluminescent formats for over 20 different targets (see list at right). For complete details, click the FACE™ Method tab below.

FACE MEK1/2 Kits provide 96 rxns each of 2 antibodies that enable you to monitor and compare the levels of both phosphorylated and total MEK1/2. The phospho-MEK1/2 antibody recognizes MEK1/2 only when phosphorylated at Ser217/Ser221, and also reacts with MEK1/2 when singly phosphorylated at Ser217 but not at Ser221. The total-MEK1/2 antibody recognizes MEK1/2 proteins regardless of their phosphorylation states. Click the MEK1/2 Info tab below for data and more information.

 
Name Format Cat No. Price  
FACE™ MEK1/2 1 x 96 rxns 48180 $525 Buy Now
FACE™ MEK1/2 5 x 96 rxns 48680 $2,100 Buy Now
FACE™ MEK1/2 Chemi 1 x 96 rxns 48280 $580 Buy Now
FACE™ MEK1/2 Chemi 5 x 96 rxns 48780 $2,320 Buy Now
Measurement of phosphorylated MEK1/2 (S217/S221) and total MEK1/2 using the FACE MEK1/2 Cell Based ELISA (In Cell Western) method.
 
Figure 1: Measurement of phosphorylated and total MEK1/2.

NIH/3T3 cells were cultured in 96-well plates and serum-starved for 16 hours. Cells were then treated with 20% FBS for 30 minutes and fixed. Total and phospho MEK1/2 were each assayed in triplicate using the phospho and total MEK1/2 antibodies included in the FACE MEK1/2 Kit. Data was plotted after correction for cell number (performed through use of Crystal Violet).

Antibody Specificities

The phospho-MEK1/2 antibody is specific for phosphorylated MEK1/2 and was raised against a synthetic phospho-peptide corresponding to residues surrounding Ser217/Ser221 of human MEK1/2. This antibody recognizes MEK1/2 only when phosphorylated at Ser217/Ser221, and also reacts with MEK1/2 when singly phosphorylated at Ser217 but not at Ser221. It does not cross-react with related kinases. The total-MEK1/2 antibody recognizes MEK1/2 proteins regardless of their phosphorylation

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