MGMT antibody (pAb), sample

by Active Motif
Available SKUs: 39952 |
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for MGMT antibody (pAb)Application Notes

Validated Applications:
IP: 10 µl per IP
WB: 1:500 - 1:1,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for MGMT antibody (pAb)Immunogen

This MGMT antibody was raised against a peptide derived from the C-terminal region of human MGMT.

for MGMT antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for MGMT antibody (pAb)References

MGMT antibody (pAb) tested by immunoprecipitation.

MGMT antibody (pAb) tested by Immunoprecipitation.
10 µl of MGMT antibody was used to immunoprecipitate MGMT from 250 µg of MCF-7 nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the MGMT antibody at a dilution of 1:500.

MGMT antibody (pAb) tested by Western blot.

MGMT antibody (pAb) tested by Western blot.
Nuclear extract of MCF-7 cells (20 µg) probed with MGMT antibody (pAb) at a dilution of 1:500.

for MGMT antibody (pAb)Background

MGMT (O-6 methylguanine DNA methyltransferase) is an enzyme involved in DNA damage repair. MGMT transfers methyl groups from O-6 methylguanine methylated DNA, and other methylated adducts, to a cysteine residue in itself, thus reversing DNA damage caused by alkylation. This is a suicide reaction as the enzyme is irreversibly inactivated. MGMT helps prevent DNA alkylation events from causing mutations. It also contributes to resistance to chemotherapy in cancer patients being treated with alkylating drugs.

for MGMT antibody (pAb)Positive Control

Active Motif's MCF-7 nuclear extract (Catalog No. 36017) can be used as a positive control for MGMT antibody.

for MGMT antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for MGMT antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

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