The ChIP-IT Control qPCR Kits include a positive control antibody, a bridging antibody to enhance binding affinity of mouse monoclonal antibodies, a negative control antibody to evaluate non-specific binding and species-specific positive and negative control qPCR primers. The positive and negative control primer sets enable direct comparison of gene targets for the same antibody immunoprecipitation. This eliminates the variation that is seen when comparing a ChIP antibody result to that of a negative control IgG antibody, as each have different binding affinities.
