Global 5-hmC DNA ELISA Kit

Active Motif's Global 5-hmC DNA ELISA Kit is designed to detect and quantify 5-hydroxymethylcytosine (5-hmC) levels in genomic DNA from samples with different disease states, treatment conditions, clinical outcomes or environmental backgrounds. Quantification of 5-hmC using the Global 5-hmC DNA ELISA Kit is consistent with mass spectrometry, while allowing for higher throughput processing of samples and requiring only nanogram amounts of genomic DNA. The kit contains an optimized protocol and all necessary reagents to perform DNA fragmentation, plate coating, capture and colorimetric detection of 5-hmC. For quantitative analysis, a synthetic DNA standard oligo containing an exact number of 5-hmC is included in the kit. Serial dilutions of the standard can be utilized to generate a standard curve for determination of both molar and % 5-hmC values in the DNA samples.