ADCC Bioassay Effector Cells, F Variant, Propagation Model
Determine the Effect of the Low Affinity F158 Fc?RIIIa Polymorphism
The ADCC Reporter Bioassay, F Variant, provides a biologically relevant and specific MOA-based measure of ADCC without the complex workflow and variability inherent in primary cell-based assays. Specifically, primary donor PBMC or NK cells are replaced with a Jurkat cell stably expressing the low-affinity F158 human Fc?RIIIa receptor and NFAT-induced luciferase. Importantly, the ADCC Reporter Bioassay, F Variant, demonstrates rank ordering of antibody activity equivalent to classic LDH release ADCC bioassays. The bioassay also can be used to quantify effects of antibody glycosylation on Fc effector function.
ADCC Bioassay Effector Cells, F Variant, Propagation Model, allows propagation and banking of the ADCC Bioassay Effector Cells. Bio-Glo™ Luciferase Assay System is the required reagent for use with ADCC Bioassay Effector Cells, Propagation Model. Product includes two vials at 2 × 107 cells/ml and 0.65ml/vial. One vial should be thawed, propagated and cells frozen to create a cell bank. The remaining vial should be reserved as backup.
ADCC Reporter Bioassay Effector Cells, F Variant, Propagation Model Technical Manual
PDF (1 MB)
Certificate of AnalysisLookup Certificate of Analysis
For Research Use Only. Not for Use in Diagnostic Procedures.