Twist Bioscience NGS | Fixed Panels | Exome 2.0
Twist Bioscience - Next Generation Sequencing - Twist Bioscience Exome 2.0
For best-in-class results, use best-in-class tools
Twist Exome 2.0 is designed to detect rare and inherited diseases, as well as germline cancers. This panel’s high uniformity and low off-target rate deliver best-in-class sequencing efficiency, enabling quality data to be collected with less sequencing. With superior coverage of major genetic databases (RefSeq, CCDS, GenCode, Clinvar, ACMG73 and more) and the addition of clinically relevant non-coding pathogenic and likely pathogenic variants, Twist Exome 2.0 provides the value of multiple clinical panels all wrapped into one, easily customizable package.
Coverage That Matters
- Covers 36.5 Mb of human protein coding regions
- Based on recent database releases
- Includes carefully curated clinical content
- Uniform enrichment of target regions
- NGS QC of all probes
- Easily spike-in content into the exome
Effective across multiplex target enrichment workflows
Run overnight or same day workflows
Twist Exome 2.0 earns the title “best-in-class” by outperforming top competitors and enabling the most efficient exome sequencing. Compared to other exome panels, Twist Exome 2.0 delivers higher uniformity and a higher on-target rate as well as lower drop out and duplicate read rates. Together, this equates to fewer wasted reads and maximal coverage of target sequences.
Most complete coverage of the human exome plus more
Twist Exome 2.0 features a complete new design that maximizes coverage of clinically relevant regions from the ClinVar database while including updated coverage of protein-coding regions from CCDS, RefSeq and GENCODE. In addition, the new design also includes pharmacogenomic SNPs, extended Tert promoter coverage, and 41 Sample ID SNPs for resolving contamination issues (Eurogentest, Pengally et al.).
Low GC bias enables target capture, regardless of GC content
Twist Exome 2.0 provides higher uniformity relative to competitors. Folded into this superior uniformity is a lower GC bias, meaning this panel can help you detect complex targets containing high, or low, levels of GC content. This means fewer rounds of sequencing are needed to adequately read targets with extreme GC content.
Most efficient exome panel
Twist Exome 2.0’s superior uniformity, on-target rate, duplicate rate, and overall coverage translates into a highly efficient, cost-saving exome panel. This efficiency enables you to sequence more samples per run, or else to achieve deeper sequencing over desired targets.
Twist Exome 2.0 is designed to help you cover the target regions that are most important to you. To do this, we’ve built it to be amenable to a wide variety of customization options. If you’re studying calls for analysis of promoters, intronic SNPs, or UTRs, custom content can be quickly and affordably spiked into the panel to provide coverage. High-throughput studies are also enabled through sample multiplexing, up to 16-plex per reaction.
Human Exome 2.0 Plus Comprehensive Exome Spike-in
Twist's latest human exome product spiked-in with all the Twist Comprehensive Exome targets that are omitted in Exome 2.0.
- Twst Exome 2.0 Panel - DOWNLOAD
- Twist Exome 2.0 .bed files
- - Change Log - DOWNLOAD
- - Twist Exome 2.0 hg19 covered targets .bed file - DOWNLOAD
- - Twist Exome 2.0 hg38 covered targets .bed file here - DOWNLOAD
- Comparison data of Twist Exome 2.0 panels with a selection of four commercially available exome panels - DOWNLOAD
- Twist Exome 2.0 Panel - DOWNLOAD
"We recently adapted the Twist Exome 2.0 for our work for various indications, especially for unexplained developmental disorders. The content, performance and quality are the best we have experienced from any provider, and we didn’t have to choose between robust content and performance." - Oliver Wachter of the Center for Human Genetics and Laboratory Diagnostics in Germany (MVZ-Martinsried GmbH)
* For research use only. Not for diagnostic procedures.