Recombinant Histone H4K20me1 (MLA), His-tag was expressed in E. coli cells as full-length protein (accession number: NP_003539.1) with a C-terminal 6×His tag, of which the Lysine 20 was mutated to Cysteine and then mono-methylated via MLA technology. The molecular weight of this protein is 12334 Da.