Recombinant human Histone H3 monomethyl Lys36 (H3K36me1) (accession number NP_003520.1) was expressed in E. coli cells and contains two substitutions (cysteine to serine at amino acid 96 and cysteine to alanine at amino acid 110). Recombinant methylated histones are specifically methylated via a chemical alkylation reaction that introduces a methyl lysine analog (MLA). The molecular weight of the protein is 15257 Daltons. Protein Sequence (a.a. substitutions in italics): ARTKQTARKS TGGKAPRKQL ATKAARKSAP ATGGVCKPHR YRPGTVALRE IRRYQKSTEL LIRKLPFQRL VREIAQDFKT DLRFQSSAVM ALQEASEAYL VGLFEDTNLA AIHAKRVTIM PKDIQLARRI RGERA
