Twist Bioscience Synthetic Controls | Liquid Biopsy | cfDNA Pan-cancer Reference Standards
Twist Bioscience Synthetic Controls - Infectious Disease
Comprehensive Cancer Variant Reference Standard for Liquid Biopsy Research
NGS-based liquid biopsy is a rapidly evolving application that requires accurate and precise reference standards. The Twist Pan-cancer Reference Standard is a high-quality, standardized control for use in the research and development of NGS-based liquid biopsy assays. This reference standard is ideal for establishing the analytical limit of detection (LoD) for specific cancer variants and as a control to track the quality of an NGS assay workflow and assess the fidelity of the assay process during research and development of such assays.
Traditional reference standards are limited in the number and variation of variants and typically use cell line-derived DNA which can carry unwanted sequence variations and variable fragment length. The Twist cfDNA Pan-cancer Reference Standard incorporates a large and diverse set of variants for research use. This reference standard utilizes accurate DNA synthesis of variants with precise control over the specific variant allele frequencies (VAF) to produce a reference standard which closely mimics the size distribution and fragmentation profile of cell-free and circulating tumor DNA enabling higher performance assay development.
This reference standard consists of synthetically designed variant sequences that mimic circulating tumor DNA (ctDNA), combined with background DNA that is derived from, and closely mimics, human-derived cell-free DNA (cfDNA). The ctDNA sequences are designed as a tiled pool of ~167 bp sequences that closely mimic natural ctDNA and cover 458 individual mutations with 132 clinically actionable variants across 84 genes associated with cancer.
- 458 unique naturally occurring cancer variants
- 132 clinically relevant variants
- Covers 84 different genes involved in cancer
- Background DNA derived from human cfDNA samples
- DNA size profile and post-sequencing profile mimic native cfDNA
- Seven individual VAF percentages to choose from
- Digital Droplet PCR verification of VAF percentages
- A convenient test set of all VAF percentages available
Comparison of Twist’s Reference standard and competitors
DNA analysis of the Twist cfDNA Pan-Cancer Reference Standard and competitors reference standards show that Twist more closely mimics native cell-free DNA providing a more true to life test result.
Post Sequencing Profile Comparison
Post sequencing library size distribution comparison between Twist and competitors show how similar Twist mimics native cfDNA samples including the jagged leading peaks.
DOWNLOADS
SKUs
104549 *Twist cfDNA Pan-cancer reference standard set, 300ng kit
104563 Twist cfDNA Pan-cancer reference standard VAF 0% (WT), 3 ug
104564 Twist cfDNA Pan-cancer reference standard VAF 0.1%,3 ug
104565 Twist cfDNA Pan-cancer reference standard VAF 0.25%, 3 ug
104566 Twist cfDNA Pan-cancer reference standard VAF 0.5%,3 ug
104567 Twist cfDNA Pan-cancer reference standard VAF 1%, 3 ug
104568 Twist cfDNA Pan-cancer reference standard VAF 2%, 3 ug
104569 Twist cfDNA Pan-cancer reference standard VAF 5%, 3 ug
105900 Twist cfDNA Pan-cancer Reference Standard 0% 300ng, 2 tubes
105901 Twist cfDNA Pan-cancer Reference Standard 0.1% 300ng, 2 tubes
105902 Twist cfDNA Pan-cancer Reference Standard 0.25% 300ng, 2 tubes
105903 Twist cfDNA Pan-cancer Reference Standard 0.5% 300ng, 2 tubes
105904 Twist cfDNA Pan-cancer Reference Standard 1% 300ng, 2 tubes
105905 Twist cfDNA Pan-cancer Reference Standard 2% 300ng, 2 tubes
105906 Twist cfDNA Pan-cancer Reference Standard 5% 300ng, 2 tubes
104176 Twist Library Preparation Kit with amp mix. Mechanical Fragmentation, 16 Samples
104177 Twist Library Preparation Kit with amp mix, Mechanical Fragmentation, 96 Samples
105094 Twist UMI Adapters - TruSeq Compatible, 96 Samples
105040 Twist UMI Adapter System - TruSeq Compatible, 16 Samples
105041 Twist UMI Adapter System - TruSeq Compatible, 96 Samples Plate A
105042 Twist UMI Adapter System - TruSeq Compatible, 96 Samples Plate B
105043 Twist UMI Adapter System - TruSeq Compatible, 96 Samples Plate C
105044 Twist UMI Adapter System - TruSeq Compatible, 96 Samples Plate D
* For research use only. Not for diagnostic procedures.