Twist Bioscience NGS | Fixed Panels | Mouse Exome Panel
Mouse Exome Panel
The mouse is an extremely important model system for studying genetic variation, tumor mutations, and phenotypic outcomes as well as the therapeutic effect of pharmaceutical agents. As genetic variant databases are continuously updated, the Twist Mouse Exome panel is thoughtfully designed and built from the most current databases. When combined with Twist’s expanding portfolio of library preparation and enrichment reagents, the complete toolset allows researchers to achieve industry-leading coverage across target regions while optimizing sequencing cost and sample throughput.
With 5-Gb sequencing, the narrow variance and mode of the coverage distribution (left) reduces both the number of bases found below the targeted level of coverage (right) and the over- and under-sequencing from the typically skewed coverage associated with poorer uniformity. This is aided by the balanced capture profile across a much wider range of target %GC content.
Picard metrics of the Twist Mouse Exome Kit with two strains of mouse. Data obtained with 5 Gb (135x) sequencing.
KEY BENEFITS
- Based on mouse genome assembly mm10
- 37.7 Mb comprehensive coverage of major gene annotation databases
- 95% of targeted regions covered at 20x with only 5Gb sequencing
- High complexity libraries for high sensitivity variant calling
SKUs
- 102035 - Twist Mouse Exome, 2 Reactions, Kit
- 102036 - Twist Mouse Exome, 12 Reactions, Kit
- 102037 - Twist Mouse Exome, 96 Reactions, Kit
- Workflow Step: Target Enrichment
- Format: dsDNA
- Panel size: 37.7 Mb
- Content: Mouse protein coding genes
- Design Databases: RefSeq
