Twist Bioscience Genes | Clonal Genes
Twist Bioscience Clonal Genes
Twist Bioscience’s platform is capable of synthesizing hundreds of thousands of genes each month to meet all your DNA needs. Their silicon-based platform for DNA synthesis enables us to create at scale, highly precise, sequence-perfect clonal genes of various lengths and difficulty that are all NGS verified and delivered on time when you need them.
Twist Bioscience Clonal Genes Benefits
Low Cost – High Quality
- No hidden sub-cloning or DNA complexity fees
- Quick delivery
DNA Your Way
- 0.3 – 5 kb genes cloned into a plasmid of your choice
- Choose a Twist Catalog Vector or send us yours
- 4 prep scales (50ng – 2µg | 2µg – 10µg | 10µg – 100µg | 100µg – 1mg)
- Normalization and endotoxin-free options are available
Scalable Synthesis
- No order limits
- Same turnaround regardless of order size
- DATA:
Sequence Perfect Genes
A graphical representation of our standard NGS verification performed on every clonal gene. The clonal gene in this figure is an example of an error-free clone. The read depth is indicated for the entire plasmid and no SNPs or indels were detected.
Clonal Genes Success Rate
Twist Bioscience’s observed success rate for clonal genes. The success rate is defined as the percentage of clonal genes ordered that were delivered without any SNPs or indels.
*T&Cs: Turnaround time for Clonal Genes is subject to change based on customizations and complexity. The average turnaround time for Clonal Genes is 10-15 business days (ex. delivery). New vector onboarding for Clonal Genes will add additional time.
DOWNLOADS
- Clonal Genes Product Sheet
- Gene Fragments Product Sheet
- Pathway Engineering Through 5kb Gene Synthesis
- Speeding Up Antibody Discovery for Infectious Disease
- Twist Stock Vectors for Clonal Gene Synthesis
- Twist Tips for Genes: How to Design Your Gene
- Twist Tips for Genes - Meeting Minimum Length Requirements for Genes in Custom Vectors
- Twist DNA Resuspension Guidelines
- From Design to Protein: Cell-Free Protein Expression Using Twist Gene Fragments
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WEBINAR: Next Generation Platforms for Strain Optimisation
FAQ
Yes, there is an additional charge.
Twist can clone gene synthesis products of up to 5,000 bp into any of our catalog vectors. We also offer cloning into your own custom vector.
No - at this time, Twist does not have vectors for expression in plants; however, we can onboard a custom vector as long as it meets our requirements.
For Gene Fragments (either with or without adapters), you may wish to add your own restriction sites onto the ends of each fragment in order to easily clone the fragment into your vector of choice.
For Clonal Genes, simply submit the unique insert sequences that you would like us to clone into the vector backbone. We use a proprietary homology-based cloning method to assemble our clonal genes and we design and add the homologous overhang sequences for you - you do not need to consider our cloning strategy when designing your insert.
Please note that Twist Cloning vectors do not have restriction sites directly flanking the insert. This allows for complete flexibility of design so that you can append the desired restriction sites onto the flanks of your insert for use in your downstream workflow.
Twist Expression vector insertion points are denoted by two restriction sites - these will be fully preserved directly flanking your insert in the final construct. We recommend that customers check the final construct sequences by clicking on the "Download sequences" button in the designer. Once an order has been submitted for manufacturing, it cannot be altered in any way.
* For research use only. Not for diagnostic procedures.
