Twist Bioscience NGS | Library Preparation | Mechanical Fragmentation Library Preparation Kit
Twist Bioscience Next Generation Sequencing Library Preparation
Twist Mechanical Fragmentation Library Preparation Kit
Twist Library Preparation Kits streamline the construction of high-quality DNA libraries for next-generation sequencing (NGS) applications. Two configurations accommodate either enzymatic or mechanical DNA fragmentation, and both are optimized to facilitate library preparation for whole genome sequencing and targeted enrichment. The kits combine library preparation steps into a single reaction for improved efficiency and consistent results.
Twist Library Preparation Kit (Mechanical Fragmentation) is designed for use
with gDNA that has already been sheared mechanically. It combines the enzymes
and reagents for end repair, dA-tailing, and adapter ligation into a single reaction.
- Accommodates a wide range of DNA input types
- Enables high-quality sequencing of low-quality samples
- Minimizes start / stop site artifacts
KEY BENEFITS
- Use enzymatic fragmentation for automated, high-throughput gDNA library construction
- Use mechanical fragmentation for DNA input of varying types and quality
- Use with either Twist full-length CD adapters for PCR-free library preparation
- Use with the Twist Universal Adapter (UDI) System for maximum yield
- Optimized for use in the Twist Targeted NGS workflow
- Flexible DNA sample input from 1 ng – 1 µg (enzymatic)
- Convert DNA samples into robust, amplified libraries in under 2.5 hours
- Combine library preparation steps into a single reaction for improved efficiency and consistent results
Twist’s Rationally Sculpted Libraries Are Unparalleled in Quality and Composition:
Comparison of Combinatorial Variant Library Generation Methods
SKUs
101280: Twist Library Preparation Kit, Mechanical Fragmentation, 16 Samples
101281: Twist Library Preparation Kit, Mechanical Fragmentation, 96 Samples
Reagents, enzymes, and purification beads required for the library construction
of mechanically fragmented gDNA.
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PRODUCT SHEETS
Twist NGS Library Preparation Kits
Twist Library Preparation Kits streamline the construction of high-quality DNA libraries for next-generation sequencing (NGS) applications. Two configurations accommodate either enzymatic or mechanical DNA fragmentation, and both are optimized to facilitate library preparation for whole genome sequencing and targeted enrichment. The kits combine library preparation steps into a single reaction for improved efficiency and consistent results. Read the product sheet to find out more.
- Key benefits of Twist NGS Library Preparation Kit
- How Twist NGS Library Preparation Kits fit into your DNA sequencing workflow
PROTOCOLS
This protocol is for the generation of amplified, indexed libraries needed for downstream target enrichment and sequencing on Illumina next-generation sequencing (NGS) systems. This Twist Library Preparation Kit provides the reagents needed to prepare genomic DNA (gDNA) libraries using mechanically fragmented gDNA with either the Twist Universal Adapter System or the Twist HT (High-Throughput) Universal Adapter System. The Twist Universal Adapter System consists of Twist Universal Adapters and Twist Unique Dual Indexed (UDI) Primers, whereas the Twist HT Universal Adapter System includes Twist Universal Adapters and HT UDI Primers. This library preparation protocol is optimized for use with Twist Target Enrichment Kits and should only be performed with reagents specified or their equivalents.
- User supplied materials and equipment
- General notes and precaution
- Guidelines for gDNA sample
- Twist Mechanical Fragmentation Library Preparation Workflow
SKUs:
| 101898 | Twist MF Library Prep + UDIs and Fast Hyb, 2 Reactions |
| 101906 | Twist MF Library Prep + CDs and Fast Hyb, 12 Reactions |
| 101902 | Twist MF Library Prep + UDIs and Fast Hyb, 12 Reactions |
| 103897 | Twist MF Library Prep + UDIs Plate B and Fast Hyb, 12 Reactions |
| 103901 | Twist MF Library Prep + UDIs Plate C and Fast Hyb, 12 Reactions |
| 103905 | Twist MF Library Prep + UDIs Plate D and Fast Hyb, 12 Reactions |
| 101899 | Twist MF Library Prep + UDIs and Standard Hyb, 2 Reactions |
| 101907 | Twist MF Library Prep + CDs and Standard Hyb, 12 Reactions |
| 101903 | Twist MF Library Prep + UDIs and Standard Hyb, 12 Reactions |
| 103898 | Twist MF Library Prep + UDIs Plate B and Standard Hyb, 12 Reactions |
| 103902 | Twist MF Library Prep + UDIs Plate C and Standard Hyb, 12 Reactions |
| 103906 | Twist MF Library Prep + UDIs Plate D and Standard Hyb, 12 Reactions |
| 104201 | Twist MF Library Prep with amp mix+ UDIs Plate D and Standard Hyb with amp mix, 12 Reactions |
| 104184 | Twist MF Library Prep with amp mix + UDIs and Fast Hyb, 2 Reactions |
| 104204 | Twist MF Library Prep with amp mix + CDs and Fast Hyb with amp mix, 12 Reactions |
| 104192 | Twist MF Library Prep with amp mix + UDIs Plate B and Fast Hyb with amp mix, 12 Reactions |
| 104196 | Twist MF Library Prep with amp mix + UDIs Plate C and Fast Hyb with amp mix, 12 Reactions |
| 104200 | Twist MF Library Prep with amp mix + UDIs Plate D and Fast Hyb with amp mix, 12 Reactions |
| 104188 | Twist MF Library Prep with amp mix + UDIs and Fast Hyb with amp mix , 12 Reactions |
| 104185 | Twist MF Library Prep with amp mix + UDIs and Standard Hyb, 2 Reactions |
| 104205 | Twist MF Library Prep with amp mix + CDs and Standard Hyb with amp mix, 12 Reactions |
| 104189 | Twist MF Library Prep with amp mix + UDIs and Standard Hyb with amp mix, 12 Reactions |
| 104193 | Twist MF Library Prep with amp mix + UDIs Plate B and Standard Hyb with amp mix, 12 Reactions |
| 104197 | Twist MF Library Prep with amp mix + UDIs Plate C and Standard Hyb with amp mix, 12 Reactions |
| 106458 | Twist MF Library Prep + Std Hyb + Dry Down Beads, 16x2 rxns |
| 106456 | Twist MF Library Prep + Std Hyb + Dry Down Beads, 96x12 rxns |
| 106666 | Twist MF Library Prep + Std Hyb v2 + Dry Down Beads, 16x2 |
| 106665 | Twist MF Library Prep + Std Hyb v2 + Dry Down Beads, 96x12 |
| 106457 | Twist MF Library Prep + Fast Hyb + Dry Down Beads, 16x2 rxns |
| 106455 | Twist MF Library Prep + Fast Hyb + Dry Down Beads, 96x12rxns |
