Twist Bioscience Genes | Gene Fragments
Twist Bioscience Gene Fragments
Synthetic Gene Fragments are an inexpensive, rapid, and efficient way to build the genes you need for your research. Gene Fragments from Twist Bioscience improve your cloning process by minimizing colony screening. This allows you to save time and money by dramatically reducing cloning and sequencing costs.
Gene Fragments by Twist Bioscience Benefits:
- Gene Fragments for assembly and cloning
- Compatible with all downstream cloning methods
- Quick delivery
- Industry-leading error rate
- Length: 300 bp – 1800 bp
- Yield: 100 ng – 1 µg
- No order limit
- DATA
Twist’s DNA synthesis technology outperforms the competition with exceptionally low error rates.
In a direct comparison of Gene Fragment products, Twist consistently showed the lowest error rate. The graph to the left represents the average error rate for Gene sequences ordered from Twist and Integrated DNA Technologies, Inc. (IDT). The results show Twist Gene Fragments have greater sequence accuracy over eBlocks and gBlocks with an average of 2-fold greater accuracy in sequence fidelity for Twist Gene Fragments over gBlocks.
More perfect Genes from Twist
Twist Gene Fragments consistently yield the highest percentage of perfect clones which saves you time and money in your research. The graph to the left shows a direct comparison of the percentage of sequence-perfect clones across a wide variety of gene lengths and sequences for 3 different gene products. Data was generated using a set of 63 sequences with a wide variety of gene lengths to reflect the diversity of gene lengths required for various real-world synthetic biology applications.
DOWNLOADS
- Clonal Genes Product Sheet
- Gene Fragments Product Sheet
- Pathway Engineering Through 5kb Gene Synthesis
- Speeding Up Antibody Discovery for Infectious Disease
- Twist Stock Vectors for Clonal Gene Synthesis
- Twist Tips for Genes: How to Design Your Gene
- Twist Tips for Genes - Meeting Minimum Length Requirements for Genes in Custom Vectors
- Twist DNA Resuspension Guidelines
- From Design to Protein: Cell-Free Protein Expression Using Twist Gene Fragments
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FAQ
What is the price for a gene fragment?
The price of a gene fragment will vary based on the size of the fragment. Fragments can be ordered with or without adapters.
What methods can I use to clone my adapter-on gene fragments?
You can use the following methods to clone gene fragments with adapters:
Restriction digestion/Ligation - place restriction sites inside of the Twist adapters for easy cloning
Type IIS or Golden Gate - place restriction sites inside of the Twist adapters for easy cloning TOPO cloning (blunt-end) - the adapter sequences will be cloned in as well
Gateway cloning - add attB sites to the ends of your sequence
Are adapter sequences appended onto the ends of my sequences?
For Adapter-On Gene Fragments, universal adapters are synthesized onto the ends of each fragment. The adapters are 21-22 nucleotides in length. Fragments with these adapters are compatible with a number of assembly methods, namely Restriction Digestion/Ligation, Gateway, Golden Gate, and TOPO cloning.
The addition of restriction sites inside of the adapters can be used to remove adapters. TOPO cloning will work but will leave the adapters on.
ADAPTER SEQUENCES
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For orders received before August 9, 2021, the adapter sequences are as follows:
- 5' Adapter: GAAGTGCCATTCCGCCTGACCT
- 3' Adapter: AGGCTAGGTGGAGGCTCAGTG
- 5’ – GAAGTGCCATTCCGCCTGACCT – insert – AGGCTAGGTGGAGGCTCAGTG – 3'
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For orders received after August 9, 2021 to November 1, 2021, the adapter sequences are as follows:
- 5' Adapter: CCCCGTCACCTTTGGCTTATCAGT
- 3' Adapter: AGTGACATCTGGACGCTAAGACCG
- 5’ - CCCCGTCACCTTTGGCTTATCAGT – insert – AGTGACATCTGGACGCTAAGACCG - 3'
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For orders received after November 2, 2021, the adapter sequences are as follows:
- 5' Adapter: CAATCCGCCCTCACTACAACCG
- 3' Adapter: CTACTCTGGCGTCGATGAGGGA
- 5’ - CAATCCGCCCTCACTACAACCG – insert – CTACTCTGGCGTCGATGAGGGA - 3'
*Adapters are short sequences added to each end of the insert as part of our synthesis process. Gene fragments without these adapters are also available during the checkout process.
Are Gene Fragments Purified?
Yes, gene fragments go through a bead-based clean up; however, short fragments can carry over in small quantities.
What are the adapter sequences used on the Adapter-On Gene Fragments?
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For orders received before August 9, 2021, the adapter sequences are as follows:
- 5' Adapter: GAAGTGCCATTCCGCCTGACCT
- 3' Adapter: AGGCTAGGTGGAGGCTCAGTG
- 5’ – GAAGTGCCATTCCGCCTGACCT – insert – AGGCTAGGTGGAGGCTCAGTG – 3'
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For orders received after August 9, 2021 to November 1, 2021, the adapter sequences are as follows:
- 5' Adapter: CCCCGTCACCTTTGGCTTATCAGT
- 3' Adapter: AGTGACATCTGGACGCTAAGACCG
- 5’ - CCCCGTCACCTTTGGCTTATCAGT – insert – AGTGACATCTGGACGCTAAGACCG - 3'
-
For orders received after November 2, 2021, the adapter sequences are as follows:
- 5' Adapter: CAATCCGCCCTCACTACAACCG
- 3' Adapter: CTACTCTGGCGTCGATGAGGGA
- 5’ - CAATCCGCCCTCACTACAACCG – insert – CTACTCTGGCGTCGATGAGGGA - 3'
