Twist Bioscience NGS | Fixed Panels | Mitochondrial Panel

A critical component for successful targeted sequencing analysis is the quality of the capture reaction, which drives the uniformity of reads. A more uniform capture leads to more uniform sequencing, as indicated by lower fold80 base penalty scores. With higher uniformity, sequencing reads can effectively drive on target coverage, limiting wasted reads. High uniformity is the combined result of the probe design algorithm, silicon-based synthesis of dsDNA probes, and calibration processes that ensure uniform amplification, GC content, and enrichment performance across all targets. NGS-based quality control of all Twist Panels ensures that all probes are present at the correct concentrations and that the reproducibility of results is independent of our production processes.

Graph shows the Twist Mitochondrial panel spiked into Twist Core Exome. Data obtained with 2.3 Mb (150X) sequencing. Complete coverage with excellent uniformity and sequencing efficiency is demonstrated.

The Twist Mitochondrial Panel can serve as added content to the Twist Human Core & Comprehensive Exome Panels, a Twist Custom Panel, or used as a standalone panel (figure 1)

Sequencing results with the Mitochondrial Panel as a spike-in with the Twist Human Core Exome at different concentrations demonstrate similar coverage indicative of uniformity irrespective of varying concentrations (figure 2).