FuGENE HD Transfection Reagent is a multi-component reagent that forms a complex with DNA, then transports the complex into animal or insect cells. It is suitable for use in media with or without serum, and for transient or stable transfection, as well as co-transfections of multiple DNA plasmids. Due to its minimal cytotoxicity, there is no need to change media after an incubation with the transfection FuGENE HD mix.