Applications Validated by Active Motif:
ChIP-Seq: 5 µg each ChIP
WB*: 0.5 - 2 µg/ml
*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
This antibody was raised against a peptide containing monomethyl-lysine 4 of Histone H3.
Rabbit serum containing 30% glycerol and 0.035% sodium azide.Histone H3K4me1 antibody (pAb) tested by ChIP-Seq
Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 15 µg of HeLa cell chromatin and 5 µg of antibody. ChIP DNA was sequenced on the Illumina NextSeq and 14.1 million sequence tags were mapped to identify Histone H3K4me1 binding sites on chromosome 1. Histone H3K4me1 antibody Cat. No. 39299 is provided in a serum format. We also offer a Protein A purified IgG format of this antibody, Cat. No. 61781.Histone H3K4me1 antibody (pAb) tested by Luminex bead-based specificity analysis.
Various recombinant Histone proteins were conjugated to Luminex beads and incubated with varying dilutions of Histone H3K4me1 antibody. Bound antibody was detected with anti-rabbit IgG-PE and read in a MagPix Luminex Instrument.
Luminex® is a registered trademark of Luminex Corporation.Histone H3K4me1 antibody (pAb) tested by Western blot.
20 µg of HeLa cell nuclear extract was run on SDS-PAGE and probed with Histone H3K4me1 antibody at 1:2000.