MCF-7 nuclear extract (Estradiol treated)
Active Motif's MCF-7 nuclear extract (Estradiol treated) was prepared from cell cultures of the human MCF-7 breast adenocarcinoma cancer cell line. The MCF-7 cell line was originally derived from a pleural effusion of a 69-year old Caucasian woman with metastatic breast cancer. MCF-7 cells are the best characterized and most commonly utilized cell lines in in vitro breast cancer studies. The MCF-7 cell line has retained several characteristics that are particular to mammary epithelium, including the ability to grow as a monolayer, form domes, and respond to hormones. These cells also display a low metastatic potential, leading to the assumption that they represent an early epithelial adenocarcinoma phenotype. These characteristics make MCF-7 cells an ideal model system to study malignant progression in relation to breast cancer. Like mammary epithelial cells, MCF-7 cells are sensitive to estrogen. These cells process estrogen, in the form of estradiol, via cytoplasmic estrogen receptors. Treatment of with estradiol induces a proliferative response by activating the expression of early and delayed hormone-responsive genes required for cell cycle progression. In addition, estradiol treatment of MCF-7 cells causes inhibition of apoptosis.