MODified™ Protein Domain Binding Kit
The MODified Histone Peptide Array* is a valuable research tool that can be used to screen antibodies, enzymes and proteins for cross-reactivity or binding interactions with histones and their post-translational modifications (PTM). The MODified Histone Peptide Arrays screen 59 acetylation, methylation, phosphorylation, and citrullination modifications on the N-terminal tails of histones H2A, H2B, H3 and H4. Each peptide array contains 384 unique histone modification combinations in duplicate, including up to four separate modifications on the same 19mer peptide, offering the most extensive coverage available for commercial arrays of similar format. This extensive coverage of histone modifications enables the study of not only individual sites, but also the effects of neighboring modifications on recognition and binding. The unique synthesis and conjugation methods ensure greater than 95% purity of bound peptides and high peptide density at each spot, which is advantageous for analysis of interaction sites with low binding constants. The simple array protocol works like a Western blot; no special equipment needed.
Active Motif offers everything needed to study histone PTM interactions.
- MODified Histone Peptide Array – peptide arrays contain 384 unique histone modification combinations. Arrays are available individually or in packs of 5 arrays. To see a complete list of the modifications available and their corresponding peptide location, please CLICK HERE.
- Array Analyze Software – free PC compatible software program to analyze spot intensities and generate Excel-based files with raw data and graphical analysis. Please download Array Analyze Software Manual for instructions regarding the installation and operation of the software. Click here to download the software program, Array Analyze Software.
Array Labeling Kits – labeling kits contain the buffers and ECL reagents needed for detection of the Histone Peptide Array (arrays are not included).
The MODified Array Labeling Kit can be used to study antibody and enzyme interactions. A positive control antibody that recognizes the c-Myc spot on the array is included. The MODified Protein Domain Binding Kit is designed to interrogate the binding specificity of His-tagged protein reading domains. A positive control tudor domain protein is included.
- Antibodies, Enzymes and Protein Domains – Active Motif offers a broad selection of histone modification antibodies, HAT, HDAC, HMT or HDM proteins and bromodomains that can be utilized in combination with the MODified Histone Peptide Array.