The Nucleosome Assembly Control DNA will prove to be a key component of your in vitro chromatin assembly reactions. The 187 bp double stranded DNA can be directly added to your purified histones or histone extracts resulting in mononucleosome formation that can be easily visualized on a gel by mobility shift. The Nucleosome Assembly Control DNA can be used as a control to validate the proper assembly of the components of your in vitro chromatin assembly reactions. Alternatively, the Nucleosome Assembly Control DNA can be used as a template to monitor assembly kinetics in the presence or absence of chromatin interacting proteins or compounds.
