RapidClean Protein Removal kit-25 rxns
In less than 5 minutes, completely remove protein from aqueous solutions of single- and double-stranded nucleic acids.
RapidClean is a novel affinity resin designed to remove all protein from aqueous solutions of nucleic acids, providing an alternative to hazardous and lengthy phenol-chloroform procedures. The RapidClean resin binds and removes protein in a 5-minute protocol that combines convenience, speed, and nucleic acid recovery rates in excess of 95%.
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Figure 1. RapidClean completely removes T4 DNA ligase. No detectable ligase activity remains in a reaction mix after exraction with RapidClean (lane 2). EcoRI digested λ-DNA (lane C, Control) was incubated for 16 hours at room temperature with T4 DNA ligase (lane 1) or with the same mixture extracted with RapidClean (lane 2). |
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Figure 2. Extraction with RapidClean completely removes Exonuclease III. ϕX174/HaeIII DNA (Lane M) was digested by a 40 min incubation at 37 °C with 10 units of Exonuclease III (lane 1), but no digestion occured when the enzyme mix was extracted twice with RapidClean before addition of the ϕX174/HaeIII DNA target (lane 2).
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Figure 3. RapidClean purifies plasmid DNA as efficiently as phenol-cholorform extractions.RapidClean purifies plasmid DNA as efficiently as phenol-cholorform extractions. Crude plasmid pellet containing pUC119 plasmid DNA was ethanol precipitated, suspended in TE buffer (lane 1), treated with RNase (lanes 2 and 5), and extracted with RapidClean once (lane 3) or twice (lane 4), or extracted with phenol-chloroform once (lane 6) or twice (lane 7). |
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Figure 4. Extraction with RapidClean completely removes DNase I, Mung Bean Nuclease, and S1 nuclease activity.Extraction with RapidClean completely removes DNase I, Mung Bean Nuclease, and S1 nuclease activity. M12mp18 ssDNA (lane 1) is completely digested by 30 minute incubations at 37°C with DNase 1 (lane 2), Mung Bean Nuclease (lane 4) or S1 nuclease (lane 6), but no residual nicking activity is observed if the reactions are first extracted twice with RapidClean (lanes 3, 5, and 7). Lane M: 1 kb DNA ladder.
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