Recombinant Xenopus laevis Histone H2A is produced in E. coli and purified using FPLC. Protein concentration was determined using the molar extinction coefficient for Histone H2A and absorbance at 280nm. The recombinant histone is >98% pure by SDS-PAGE. The molecular weight of the protein is 13,950 Daltons. Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points.
