Recombinant Histone H4K20me3 (MLA), His-Tag was expressed in E. coli cells as full-length protein (accession number: NP_003539.1) with a C-terminal 6×His tag, of which the Lysine 20 was mutated to Cysteine and then tri-methylated via MLA technology. The molecular weight of this protein is 12362 Da.