Recombinant Mononucleosomes H3K79me3 (MLA) - biotin consist of a 167 bp of 601 DNA with a 5'- biotin tag and two molecules each of histones H2A that includes amino acids 1-130 (end) (accession number NM_003512), H2B that includes amino acids 1-126 (end) (accession number NM_003518), H3 trimethyl Lys79 (H3K79me3) (MLA) that includes amino acids 1-136 (end) (accession number NP_003520.1) with two substitutions (cysteine to serine at amino acid 96 and cysteine to alanine at amino acid 110), and H4 that includes amino acids 1-103 (end) (accession number NM_003548). All of these histones were expressed in E. coli cells. The molecular weight of histone octamer is 108 kDa.
