TAM-ChIP Assay Reagents
Active Motif's TAM-ChIP technology is a robust chromatin immunoprecipitation (ChIP) method that combines the antibody-directed protein targeting of ChIP and Next-generation sequencing (NGS) library preparation into one step. TAM-ChIP utilizes the specificity of a ChIP-seq validated antibody to target histone or transcription factor binding sites and a TAM-ChIP anti-species antibody conjugate in combination with a unique TsTn5 transposase to deliver barcoded adapter sequences directly into the chromatin surrounding the binding site. These adapter sequences are necessary for library amplification and sequencing using Illumina platforms. By incorporating the library preparation into the ChIP reaction, you avoid sample loss commonly associated with the multiple end-repair and ligation steps of traditional library preparation methods. For more complete information, please visit the Active Motif website.