Twist Bioscience Variant Libraries | Spread out Low Diversity Libraties
The Latest Tool for Mapping Protein Sequences
Spread-Out Low Diversity (SOLD) libraries provide a quick and precise tool for mapping protein sequences to explore the complex relationship between a protein and its environment.
SOLD Libraries are a time- and money-saving tool for researchers who want to efficiently investigate combinatorial possibilities.
A library of precise combinatorial variants with uniform amino acid distribution, balanced codon usage and the ability to avoid unwanted restriction sites, provides the ultimate tool to explore the variant space. All SOLD Libraries are NGS-verified, cloning-ready, and created using Twist’s patented silicon-based synthesis platform, ensuring low error rates.
A High-Fidelity Synthetic Construct
Twist’s SOLD Libraries have shown their ability to consistently deliver amino acid distributions that closely match the requested amino acid frequencies, with a uniform distribution of variants and no dropouts.
SOLD Library: Diversity is scattered along the WT sequence
Twist's SOLD Libraries have the benefit of precisely incorporating diversity across a wild-type sequence without restricting that diversity to small variant domains. This ability to simultaneously investigate multiple amino acid positions across a sequence allows researchers to investigate, and optimize, the activity of a protein both rapidly and effectively