Spike-in Antibody

ChIP Spike-in Normalization Strategy can be used to normalize ChIP qPCR and ChIP-Seq data to reduce the effects of technical variation and sample processing bias. ChIP is a multi-step process in which variations caused by sample loss, uneven sequencing read depth or user differences can lead to results that are difficult to interpret. Active Motif' Normalization Strategy can be applied across samples and antibodies to eliminate this bias and reveal any latent biological effects induced by causal agents such as inhibitory compounds or mutants. ChIP normalization can be implemented simply by integrating our Spike-in reagents into your standard ChIP protocol. A standard ChIP reaction is set up using experimental chromatin and an antibody of interest. Active Motif' Spike-in Chromatin (Catalogue No. 53083) and Spike-in Antibody (Catalogue No. 61686) are also added, or 'spiked in', to the ChIP reaction as a minor fraction of the IP reaction. Any variation introduced during the ChIP reaction will also occur with the Spike-in chromatin. Because the amount of Spike-in Chromatin is consistent across all samples, a normalization factor to be created based on the Spike-in signal and applied to the sample genome. For more complete and up-to-date information, please visit the Active Motif website.