The peptide corresponding to amino acids 306 - 416 that contains the bromodomain sequences of BRD3 (accession number NM_007371.3) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag with an observed molecular weight of 19.2 kDa. It shows binding specificity for acetylated H4K8 and H4K12/K16/K20, as well as acetylated GATA1. Bromodomain-containing protein 3 (BRD3), also known as RING3L, belongs to the BET subclass of proteins characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as æreaders' of these epigenetic histone marks and regulate chromatin structure and gene expression. Altered expression levels of BRD3 have been observed in certain cancers. Recombinant BRD3 (306-416) is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
