Recombinant JMJD2D / KDM4D (accession number NP_060509.2) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 63.2 kDa. KDM4D (lysine (K)-specific demethylase 4D), also known as JMJD2D (Jumonji Domain Containing 2D) is a protein that functions as a histone demethylase that preferentially demethylates di- and trimethylated lysine 9 residues of histone H3, while it has no activity on monomethylated H3K9 residues.
