Recombinant Mononucleosomes (H3.3) - biotinylated

Recombinant Nucleosomes (H3.3) - biotinylated, Human consists of 5000 bp of DNA (plasmid) and two molecules each of histones H2A that includes amino acids 1-130 (end) (accession number NM_003512), H2B that includes amino acids 1-126 (end) (accession number NM_003518), H3.3 that includes amino acids 1-136 (end) (accession number NM_005324) with a C-terminal Biotin tag, and H4 that includes amino acids 1-103 (end) (accession number NM_003548). Recombinant Nucleosomes (H3.3) - biotinylated, Human was generated in E. coli cells and has an observed molecular weight of 110 kDa. Nucleosomes comprise the smallest subunit of chromatin; they consist of 147 bp of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). Therefore, nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for use in in vitro studies. More importantly, some histone methyltransferases are significantly more active, as well as specific, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes.