Recombinant Mononucleosomes H3.3 (K27M) – biotinylated, consist of a 167 bp of 601 DNA with 5' biotin tag and two molecules each of histones H2A that includes amino acids 1-130 (end) (accession number NP_003503.1), H2B that includes amino acids 1-126 (end) (accession number NP_003509.1), H3.3 that includes amino acids 1-136 (end) (accession number NP_002098.1) with a point mutation Lys27Met, and H4 that includes amino acids 1-103 (end) (accession number NP_003539.1). All of these histones were expressed in E. coli cells. The molecular weight of histone octamer is ~108 kDa. The recombinant protein is ?95% by SDS-PAGE gel.
