Recombinant PRKCE protein

Background
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PRKCE (Protein Kinase C Epsilon), a calcium-independent, phospholipid- and diacylglycerol (DAG) dependent serine/threonine-protein kinase that plays essential roles in the regulation of multiple cellular processes linked to cytoskeletal proteins, such as cell adhesion, motility, migration and cell cycle, functions in neuron growth and ion channel regulation, and is involved in immune response, cancer cell invasion and regulation of apoptosis. PRKCE mediates cell adhesion to the extracellular matrix via integrin-dependent signaling, by mediating angiotensin-2-induced activation of integrin beta-1 (ITGB1) in cardiac fibroblasts and phosphorylates MARCKS, which phosphorylates and activates PTK2/FAK, leading to the spread of cardiomyocytes. PRKCE involves in the control of the directional transport of ITGB1 in mesenchymal cells by phosphorylating vimentin (VIM), an intermediate filament (IF) protein. In epithelial cells, PRKCE associates with and phosphorylates keratin-8 (KRT8), which induces targeting of desmoplakin at desmosomes and regulates cell-cell contact. Diseases associated with PRKCE include Ischemia and Anxiety.

Application Notes
Recombinant PRKCE protein is suitable for use in in enzyme kinetics, inhibitor screening, and selectivity profiling.

Protein Details
Recombinant PRKCE protein was expressed in baculovirus expression system as the full-length protein (accession number NP_005391.1) with a N-terminal FLAG tag. The molecular weight of the protein is 85 kDa.