Recombinant SETD2 (1392-2564) (accession number NP_054878.5) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 134 kDa. SETD2 (SET Domain Containing 2) is a histone methyltransferase that methylates histone H3 at lysine 36. H3K36 methylation is associated with active chromatin. SETD2 also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA pol II. Recombinant SETD2 (1392-2564) includes amino acids 1392-2564 that contains the SET domain of SETD2 that catalyzes methylation of lysine residues.
