Twist Bioscience NGS | Library Preparation | 96-Plex Library Preparation Kit
Twist Bioscience Next Generation Sequencing Library Preparation
Twist 96-Plex Library Preparation Kit
Preparing DNA sequencing libraries often involves complex workflows and only produces small batches of samples at a time. For genomic studies where hundreds of specimens are being evaluated, cost-effective tools that can be integrated with existing processes are essential.
The Twist 96-Plex Library Preparation kit enables high-throughput library construction for whole genome sequencing. The kit is purpose-built for use in high throughput applications, including industrial seed selection, cattle breeding, and rational drug design. With primer tunability for varying GC content and up to 960 samples per kit, the 96-Plex Library Preparation kit offers an optimal balance between scalability and robust library construction.
Economical solution for hundreds of samples
Twist 96-Plex Library Preparation kit scales up without breaking the bank.
Each preparation process produces 96 sequencer-ready libraries that seamlessly integrate with existing sequencing workflows.
Workflow
The Twist 96-Plex Library Preparation workflow starts with up to 96 individual samples in a plate. After the first random priming and barcoding step, all samples are pooled into a single tube for the rest of the process. An optional plate barcode can be added in the index read position to allow simultaneous sequencing of multiple sample plates.
Recommended Application
- Low-pass sequencing
- Industrial seed selection
- Cattle breeding
- Plasmid sequence verification
Kit Overview
The Twist 96-Plex Library Preparation kit contains all reagents needed to prepare double-stranded libraries with full-length adapters compatible with Illumina sequencing chemistries from 50 nanograms of high molecular weight input material. No additional primers, enzymes, or beads are required.
The kit is available in 960-sample format, containing sufficient materials for five or ten 96-sample pooled batches.
KEY BENEFITS
- Up to 960 individually barcoded samples per kit
- Highly multiplexed reactions for up to 96 samples at once
- Low price
- No fragmentation, repair, or ligation steps necessary
- Enables optimization for high- or low-GC content samples
- The same kit can be used for multiple sample types
DATA
GC content is variable with different organisms, which can cause varying biases in library preparation. The Twist 96-Plex Library Preparation kit supplies two primer sets, for high and low GC genomes. By using the primer set suitable for the organism being tested, users can tune the kit to fit their needs.
Sequencing libraries prepared with the Twist 96-Plex Library Preparation kit display uniform read counts across each plate. Bead-based size selection evenly captures fragments within a specified range.
WEBINAR
Connecting Plant Trait Genotypes to Phenotypes using WGS Skim-Seq
DOWNLOADS
- TWIST 96-Plex Library Preparation Kit : Sample Demultiplexing Guide - DOWNLOAD
- Twist 96-Plex Library Preparation Protocol - DOWNLOAD
- Twist 96-Plex Ultra High-Throughput Rapid DNA Library Preparation for Next Generation Sequencing - DOWNLOAD
- Twist 96-Plex High Throughput NGS Library Prep for Genotyping in Populations
- GWAS in a Rat Model of Temperament Identifies Multiple Loci for Exploratory Locomotion and Anxiety-Like Traits - DOWNLOAD
SKUs
- 106541 - Twist 96-Plex Library Preparation Kit, 5x96 samples – Set A
- 106542 - Twist 96-Plex Library Preparation Kit, 5x96 samples – Set B
- 106543 - Twist 96-Plex Library Preparation, 10x96 samples
* For research use only. Not for diagnostic procedures.
