Recombinant EZH2 protein complex

The EZH2 protein complex, active contains EZH2, EED and Suz12 which are the minimal components required for histone methyltransferase activity. EZH2 is specific for H3 lysine 27, a mark of repressed regions of chromatin. Recombinant human EZH2 (accession number NM_004456, amino acids 2-end with an N-terminal His tag, molecular weight 86 kDa) was coexpressed in a baculovirus expression system along with human EED (accession number NM_003797, amino acids 2-end with an N-terminal FLAG tag, molecular weight 51 kDa) and human Suz12 (accession number NM_015355, amino acids 2-end with an N-terminal His tag, molecular weight 87 kDa). EZH2, EED and Suz12 are present in the PRC protein complexes that function as mediators of epigenetic transcriptional silencing. EZH2 _ (Enhancer of Zeste homolog 2, also designated Enx1 and SETDB1) is a human homolog of the Drosophila Enhancer of Zeste protein. It contains a SET domain that catalyzes the >mono-, di- andtrimethylation of Histone H3 Lys27. Polycomb group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. Deregulation of EZH2 is linked to a variety of cancers.