Recombinant Mononucleosomes H4K20me3 (MLA) - biotin consist of a 167 bp of 601 DNA with a 5'- biotin tag and two molecules each of histones H2A that includes amino acids 1-130 (end) (accession number NM_003512), H2B that includes amino acids 1-126 (end) (accession number NM_003518), H3 that includes amino acids 1-136 (end) (accession number NP_003520.1), and H4 trimethyl Lys 20 (H4K20me3) (MLA) that includes amino acids 1-103 (end) (accession number NM_003548). All of these histones were expressed in E. coli cells. The molecular weight of histone octamer is 108 kDa.
