As we approach the Christmas & New Year period, we’d like to notify you of our final date for receiving routine orders to facilitate delivery before the Christmas break as Friday 15th December 2023.
The MyBio Team would like to take this opportunity to thank you for your business and continued support. We look forward to meeting you again in 2024.
Recombinant human PHD3 (EGLN3) protein was expressed in E. coli as the full-length protein (accession number NP_071356.1) with an N-terminal 6× His tag. The molecular weight of the protein is 31.1 kDa. Assay Conditions: 3 uM HIF1A (HIF-1a) peptide (DLDLEALAPYIPADDDFQL) was incubated with 300 nM PHD3 protein in 30 ul reaction system containing 20 mM Tris-HCl pH 7.5, 5 mM KCl, 1.5 mM MgCl2, 1 mM DTT, 100 uM 2-oxoglutarate, 100 uM ascorbate and 50 uM (NH4)2Fe(SO4)2·6H2O for 2 hours at 30°C. MALDI-TOF was used for detection.